Coding

Part:BBa_J100107

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-03-12)


E. coli adhE gene

We have taken wt adhE gene from E. coli and redesigned it to remove internal restriction sites. We also added BsaI site to cut off the start codon so it can be ligated to C-Dog RBSs using Golden Gate Assembly (GGA). This gene was synthesized for us by GeneArt. Codon optimized for E. coli. BioBrick prefix and suffix included in synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2679
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
    Illegal PstI site found at 2694
    Illegal NotI site found at 2687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2680
    Illegal PstI site found at 127
    Illegal PstI site found at 462
    Illegal PstI site found at 2028
    Illegal PstI site found at 2617
    Illegal PstI site found at 2694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1701


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